Influence of PCOS in Obese vs. Non-Obese women from Mesenchymal Progenitors Stem Cells and Other Endometrial Cells: An in silico biomarker discovery.

Hussain Al Ssadh,Amjad Hussain Asangani,Iftikhar Aslam Tayubi,Ashvini Desai,Inamul Hasan Madar

doi:10.6026/97320630013111

Abstract

Polycystic ovary syndrome (PCOS) is endocrine system disease which affect women ages 18 to 44 where the women’s hormones are imbalance. Recently it has been reported to occur in early age. Alteration of normal gene expression in PCOS has shown negative effects on long-term health issues. PCOS has been the responsible factor for the infertility in women of reproductive age group. Early diagnosis and treatment can improve the women’s health suffering from PCOS. Earlier Studies shows correlation of PCOS upon insulin resistance with significant outcome, Current study shows the linkage between PCOS with obesity and non-obese patients. Gene expression datasets has been downloaded from GEO (control and PCOS affected patients). Normalization of the datasets were performed using R based on RMA and differentially expressed gene (DEG) were selected on the basis of p-value 0.05 followed by functional annotation of selected gene using Enrich R and DAVID. The DEGs were significantly related to PCOS with obesity and other risk factors involved in disease. The Gene Enrichment Analysis suggests alteration of genes and associated pathway in case of obesity. Current study provides a productive groundwork for specific biomarkers identification for the accurate diagnosis and efficient target for the treatment of PCOS.

Highlights

Polycystic ovary syndrome (PCOS) is one of the most common metabolic, hormonal and reproductive disorders among women of the reproductive age
Because inflammation and metabolic and endocrine abnormalities prevail in women with PCOS, the purpose of the current study was to determine whether the gene expression profile of specific endometrial cell populations, including Endometrial mesenchymal stem cells (eMSCs), in PCOS endometrium could give insights into the origin of endometrial abnormalities and sub fertility common in PCOS women
Obesity and PCOS are linked as a result of a number of ways major ones include an altered lipid profile, excess adipose tissues – mass /cells; lymph tissues, increased ability to absorb fat, excess androgen hormone levels, use of psychiatric medications, altered hormonal profile and its link with brain, mainly hypothalamus, increased insulin resistance and its association with cAMP signaling, PI3K pathway and involvement of endometrial cells of obese PCOS women in other significant pathways, for example, Wnt, Shh, Etk etc

Summary

Introduction

Polycystic ovary syndrome (PCOS) is one of the most common metabolic, hormonal and reproductive disorders among women of the reproductive age. The morbidities of PCOS are linked to various associated disease or disorders, which is not limited to obesity, insulin resistance, Type II Diabetes Mellitus, cardiovascular diseases, infertility, cancer and psychological wellbeing [3]. One such longterm morbidity was studied with reference to obesity. The symptoms are characterized by anovulation, hyperandrogenism and polycystic-appearing ovaries, are the most common endocrine disorder among reproductive-age women and are a leading cause of female infertility [4]. Anovulation and impaired oocyte maturation are the main causes of decreased fecundity in PCOS, several abnormalities in PCOS endometrium have been reported, including aberrant steroid

Objectives

Methods

Discussion

Conclusion