Influence of partial hepatectomy with hepatic artery resection on liver regeneration and hepatocyte apoptosis in rats after obstructive jaundice

Abstract

Objective To study liver regeneration and hepatocyte apoptosis in the obstructive jaundice liver after partial hepatectomy (PH) at different volumes with hepatic artery resection (HAR).Methods One hundred fifty five male SD rats which underwent common bile duct ligation (CBDL) for 5 days were randomized into following groups:the bile duct obstruction and reperfusion of bile flow (BDO-RBF) group,42% PH with BDO-RBF group,70% PH with BDO-RBF group,42% PH with HAR and BDO-RBF group and 70% PH with HAR and BDO-RBF group.The levels of HGF,bcl-2 mRNA and protein,hepatocyte proliferation/apoptosis index in hepatic tissues were dynamically observed after operation (24 h,72 h and 7 d).Mortality was calculated.Results Compared with other groups,hepatocyte proliferation /apoptosis index and the HGF,bcl-2 mRNA content of liver tissue in 70% PH with HAR group were aggravated and had a significantly increased mortality on in rats after obstructive jaundice (P<0.05).Conclusion (1)Both the liver regeneration and apoptosis are influenced by conspicuous bilirubinemia,hepatectomy and hepatic artery resection in rats after obstructive jaundice,especially by the liver resection volume and conspicuous bilirubinemia.(2) Under the condition of conspicuous bilirubinemia,the influence of 70% PH with HAR on liver regeneration and apoptosis is severer than its counterpart of 42% in the rats.Thus,the former is not safe and should be avoided and the later can be used. Key words: Liver regeneration; Obstructive Jaundice; Hepatectomy; Hepatic Artery Resection; Apoptosis