Abstract
This work details the investigation of the influence of organo-mineral supplementation on the production of secondary metabolites in in vitro-germinated Bromelia balansae Mez. The seeds were germinated in vitro and transplanted to standard soils to undergo organo-mineral supplementation. The fully-grown plants were subjected to qualitative phytopharmacological prospection; quantitative analysis of total phenols and flavonoids; as well as chemical profiling by mass spectrometry. The in vitro germination of B. balansae was successful, with a germination rate of 94.95 %, thereby equaling to “super seeds” reported in literature. Moreover, the organo-mineral supplementation enhances the production of secondary metabolites in both leaves and roots, what therefore leads to higher yield of total phenols and flavonoids. Furthermore, mass spectrometry analysis confirmed the results from phytopharmacological prospection, and allowed the detection of fragments of secondary metabolites from shikimate/polyketide/mevalonate pathways.
Highlights
The understanding of the growth and development of plants is essential to shed light on the underlying mechanisms regarding secondary metabolite production and for phytopharmacological prospection (Jamwal et al, 2018)
In vitro germination The germination rate of B. balansae was of 94.95% and the plants grew uniformly
This work detailed the investigation of the influence of organo-mineral supplementation on the production of secondary metabolites in in vitro-germinated B. balansae
Summary
The understanding of the growth and development of plants is essential to shed light on the underlying mechanisms regarding secondary metabolite production and for phytopharmacological prospection (Jamwal et al, 2018). In this regard, many authors highlighted the effects of exogenous factors on vegetal metabolism and development, such as nitrogen and phosphate supplementation (i.e. organic supplementation) as well as the use of mineral macro and micronutrients (e.g. potassium, calcium and others) (Baliza et al, 2012; Bem Junior et al, 2019; da Silva et al, 2017). The sheer chemical diversity of secondary metabolites leads to their pharmacognostic classification according to their source-metabolic pathway or shared structural features (Isah, 2019)
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