Influence of Operating Modes for the Alternating Anoxic/Oxic Process on Biological Nitrogen Removal and Extracellular Polymeric Substances of Activated Sludge

Abstract

Nitrogen removal, extracellular polymeric substances (EPS), and the chemical composition (protein (PN), polysaccharide (PS), and DNA) by the aerobic/anoxic (O/A) and the anoxic/aerobic (A/O) modes were studied in a sequencing batch reactor (SBR) fed with domestic wastewater. The results showed that the removal rates of NH4+-N were 97.5% and 98.0% in the two operating modes, respectively, and a removal efficiency of NH4+-N with high efficiency and stability was obtained. The nitrification rate was positively correlated with the nitrogen loading ratio. The influence of operating modes for the alternating anoxic/oxic mode on extracellular polymeric substances of activated sludge was evaluated. The EPS constituent in the A/O mode was slightly higher than the O/A mode. The operating mode had no effect on the contents of PN, PS, and DNA in tightly bound EPS (TB-EPS) and TB-EPS. However, PN and PS in loosely bound EPS (LB-EPS) and LB-EPS in the A/O mode were 1.38 to 1.56 times those of the O/A mode. In the two operating modes, PSs were the main constituents in the TB-EPS and EPS, while PNs were the main constituents in LB-EPS. The EPS content had a good linear correlation with the sludge settling performance.