INFLUENCE OF NONIONIC SURFACTANTS ON BACTERIOPHAGE INFECTIONS. I. EFFECT OF TRITON WR-1339 ON T2 COLIPHAGE INFECTION.

Abstract

Rose, Michael J., Jr. (Veterans Administration Hospital, Washington, D.C.), Stephen A. Aron, and Bernard W. Janicki. Influence of nonionic surfactants on bacteriophage infections. I. Effect of Triton WR-1339 on T(2) coliphage infection. J. Bacteriol. 87:933-938. 1964.-Plaques formed by T(2) phage on cultures of Escherichia coli strain B from nutrient broth which contained 0.5% Triton WR-1339 were considerably smaller than those formed on cultures from control broth. Pretreatment of phage particles with Triton did not result in small plaques. Results of population studies showed that treated and control cultures had the same growth rates. Similarly, characteristic physiological reactions of treated cultures were the same as those of the controls. Investigation of the phage growth cycle demonstrated that neither the adsorptive phase nor the latent period was affected by cultivation of the host in Triton. However, the burst size of treated cultures was significantly less than that of control cultures. Analysis of the one-step growth curve data showed that Triton did not inhibit bursting of infected cells. It was concluded that the smaller plaque size was a reflection of a reduced yield of mature virulent phage particles from the Triton-treated cultures.