Influence of non-ionizing radiation on protein metabolism in chickens

Abstract

In practical terms, the idea of using an artificial magnetic field is of particular interest, which corresponds in its physical characteristics to the geomagnetic field of the Earth to combat the negative effects of hypogeomagnetic field. Further development of this idea is associated with the use and selection of hypo-, hypermagnetic fields acting on the body with experimental pathology. In this regard, the issue of influence of different duration of irradiation with an alternating pulsed electromagnetic field of ultra-low frequency (APEMF ULF) on the indicators that characterize metabolic processes in the body is insufficiently clarified. Therefore, the aim of research has been to study the effect of alternating pulsed electromagnetic field of ultra-low frequency on protein content and protein metabolism in the body of experimental chickens of the Dominant D959 cross. For this purpose, four experimental and control groups of 120-day-old chickens have been formed – 20 heads in each. The poultry has been kept in a specially equipped room with an alternating pulsed electromagnetic field of ultra-low frequency. The total protein content has been determined by the biuret method; protein fractions (albumins, globulins: alpha-1, alpha-2, beta, gamma) – by diffuse polyacrylamide gel (PAAG) electrophoresis; the content of creatinine, urea and uric acid has been performed by spectrophotometry using standard techniques. According to the results of research, it has been established that by selecting different regimens and duration of action of APEMF ULF, it is possible to influence protein metabolism in the body of chickens. Thus, on the 80th day of continuous irradiation of experimental chickens with APEMF ULF, regardless of the level of protein in the diet, in their blood revealed an increase in total protein, the relative content of globulin fraction mainly due to γ-globulins, and an increase in creatinine, urea and uric acids. When the period of continuous irradiation has been increased to 5 months, a negative effect on protein metabolism has been revealed, which has been manifested by a decrease in total protein content, relative albumin content, decrease in creatinine, urea and uric acid in the serum of experimental chickens. The combination of long-term (for 150 days) daily 60 minutes with weekly intervals of irradiation of chickens with APEMF ULF and their feeding with a 15% increase in protein levels in the diet caused a stimulating effect on protein metabolism and resistance of experimental chickens, with increasing, the relative content of globulins due to the γ-globulin fraction, as well as the main indicators of protein metabolism – creatinine, urea, uric acid. Key words: electromagnetic influence, chicken of Dominant D959 cross, total protein, protein fractions, creatinine, urea, uric acid.