Abstract
Objective To study the effects of nicotine on osteogenic activity of osteoblasts in vitro and on osteogenic activity factor in vivo.Methods The osteoblasts were co-cultured with different concentrations of nicotine ( 1 × 10-4 mol/L).The suppressive effect of nicotine on osteoblasts was examined by methyl thiazol tetrazolium (MTT) assay.The concentrations of bone morphogenetic protein-2 (BMP-2)and alkaline phosphatase (ALP) were tested by enzyme linked immunosorbent assay (ELISA) in mice.The osteopontin ( OPN),ALP,type 1 collagen ( COL1 ) mRNA levels in the mouse femur were detected by reverse transcription-polymerase chain reaction (RT-PCR).Results Nicotine obviously suppressed the proliferation of osteoblasts.The BMP-2 and ALP levels were decreased in mice after treated by nicotine.RT-PCR revealed that OPN,ALP and COL1 mRNA levels were down-regulated in the mouse femur.Conclusion The nicotine can suppress the osteogenic activity of osteoblasts and induce the decrease of osteogenic activity factor in mice. Key words: Nicotine; Osteopontin; Osteoblast
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