Influence of Nd:YAG laser on the penetration of a bioceramic root canal sealer into dentinal tubules: A confocal analysis.

Ricardo Scarparo Navarro,Daniela De Fátima Teixeira Da Silva,Raquel Virgínia Zanetti,Adriano Fonseca De Lima,Yogendra Kumar Mishra,Anna Carolina Ratto Tempestini Horliana,Rodrigo Jardim Del Monaco,Marcelo Tavares De Oliveira

doi:10.1371/journal.pone.0202295

Ricardo Scarparo Navarro, Daniela De Fátima Teixeira Da Silva + Show 6 more

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https://doi.org/10.1371/journal.pone.0202295

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Abstract

ObjectiveThe aim of this in vitro study, is to evaluate the penetration of a bioceramic root canal sealer into dentinal tubules at 3 mm and 5 mm from the apex after Nd:YAG laser irradiation.MethodsForty freshly extracted human mandibular premolars were prepared using Reciproc® and irrigated with 17% ethylenediaminetetraacetic acid (EDTA). Teeth were divided into 4 groups: group 1, obturated with control sealer (AH Plus®); group 2, obturated with bioceramic sealer (Endosequence BC Sealer®); group 3, Nd:YAG laser + control sealer (AH Plus®); and group 4, Nd:YAG laser + bioceramic sealer (Endosequence BC Sealer®). The samples were transversely sectioned 3 mm and 5 mm from the apex and examined using confocal laser scanning microscopy. Two parameters were measured: 1) sealer penetration into dentinal tubules of the root canal and 2) sealer penetration into the perimeter of the root canal walls.ResultsPenetration analysis showed that bioceramic sealer had a higher penetration at depths of 3 and 5 mm than that of the control sealer, regardless of laser use (p <0.05). Perimeter analysis showed that there was no difference between both sealers at a depth of 3 mm (p <0.05), regardless of laser use. At a depth of 5 mm, bioceramic sealer and laser showed a greater perimeter of penetration (p <0.05) than the control sealer.ConclusionThe use of Nd:YAG laser did not compromise the penetration of bioceramic sealer into dentinal tubules of root canals at 3 mm and 5 mm from the apex.

Highlights

Mechanization of root canal instrumentation has made endodontic treatment more precise, efficient, and fast [1]
Penetration analysis showed that bioceramic sealer had a higher penetration at depths of 3 and 5 mm than that of the control sealer, regardless of laser use (p
The use of Nd:YAG laser did not compromise the penetration of bioceramic sealer into dentinal tubules of root canals at 3 mm and 5 mm from the apex

Summary

Introduction

Mechanization of root canal instrumentation has made endodontic treatment more precise, efficient, and fast [1]. Instrumentation alone cannot completely decontaminate the root canals [4,5,6], making auxiliary therapies important. Several auxiliary techniques, such as high power lasers, photodynamic therapy, new instruments, and new irrigation approaches have been used to decontaminate dentinal tubules [5,7,8,9,10,11,12,13,14,15]. Despite its importance in cleaning and disinfection, the sealing of dentinal tubules could be a disadvantage because it may interfere with the penetration of sealers into root canals [19,20,21,22]

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