Abstract
In this study, we investigated the influence of the fortification of soy milk with NaFeEDTA on blood hemoglobin levels in male Sprague Dawley rats (Rattus norvegicus L.). Using a completely randomized design, 25 rats were divided into five groups. A normal control group was administered standard feeding without the addition of soy milk and fortificant, whereas a treatment control group was administered soy milk without fortificant; three treatment groups were administered soy milk fortified with the following doses of NaFeEDTA: 2.7 mg Fe/kg bw (treatment group 1), 5.4 mg Fe/kg bw (treatment group 2), and 10.8 mg Fe/kg bw (treatment group 3). The five groups were consecutively treated for 21 days. Rat blood hemoglobin measurements were performed using a hematology analyzer. The results of one-way ANOVA and least significant difference tests demonstrated that after the 21-day consecutive treatment, there was a significant influence on the blood hemoglobin levels in all treatment groups when compared with treatment group 1, whereas treatment groups 2 and 3 exerted a significant influence on the blood hemoglobin levels when compared with the treatment control group. The highest increase in the blood hemoglobin levels was detected in treatment group 2, which was a 10.84 % increase from the normal control group and 9.28 % increase from the treatment control group.
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