Abstract
This study investigated the effects of ultrasonic treatment with different intensities (200, 400, and 600 W) and durations (15, 30, and 45 min) on the mycelial integrity, product quality, and in-vitro digestibility of mycoprotein. Results showed that the mycelium was damaged to varying degrees under different ultrasonic parameters. The mycelium showed obvious damage when the time of ultrasonic treatment lasted more than 30 min or the power exceeded 400 W. As a result, the particle size, hardness and chewiness of mycoprotein products were decreased significantly. Compared with the control group (CK), ultrasonic treatment increased the proportion of immobile water and changed the secondary structure of the fungal intracellular protein, which may be the reason for improving in-vitro digestibility of mycoprotein products. This result demonstrated that, by damaging the mycelial integrity and changing the protein secondary structure, ultrasound treatment could decrease the texture characteristics, but improve the water-holding capacity and in-vitro digestibility of products. This study would provide a potential application for the minced meat products by mycoprotein treated with ultrasound.
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