Abstract
Tris(1,3-dimethyphenylcarbamate)-derivatized cellulose, bonded to silica, was used as a chiral stationary phase for the high performance liquid chromatographic separation of optically active potential drug substance and corresponding enantiomer over the temperature range of 12 to 40 in the reversed-phase mode. A decrease in temperature caused an increase in the retention for solutes. The van't Hoff plots generated using acetonitrile/acetate buffer mobile phase system were nicely linear, thus, we calculated enthalpies and entropies of solute transfer from the mobile to stationary phase. The influence of the mobile phase composition was studied in detail. The cavity formation effect was the major factor that governs the solute distribution between the mobile and stationary phases for acetonitrile-rich mobile phase, while the effect of solvation due to acid–base equilibrium became significant in highly aqueous mobile phase. Thermodynamic data also revealed that enantio-separation was enthalpy-controlled separation on a chiral cellulose-derivatized column under iso-enantioselective temperature.
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