Abstract
The dependences of retention data, expressed as capacity factors k′, linear capacity of the column θ 0.1, height equivalent to a theoretical plate (HETP) and peak shape, on the sample size are described. The measurements were carried out on a silica stationary phase with twelve different mobile phases. Cyclohexanol and cyclohexanone were the injected samples. It was found that the shape of the adsorption isotherm and consequently the symmetry of the sample elution peak is determined by the composition of the mobile phase. Also, the retention times decrease or increase with size of the injected sample depending on the adsorption type, i.e., depending on the sample type and the mobile phase. A suitable mobile phase composition may result in an expansion of the region of the linear capacity of the column.
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