Influence of miR -203 on growth and proliferation of glioma cell line U251 by targeting survivin

Abstract

Objective To investigate the influence of miR -203 on growth and proliferation of human glioma cell line U251.Methods Target of miR - 203 was forecasted by bioinformatics.U251cells were transfected with mimics or expression plasmid of miR - 203 using Lipofectamine 2000. After transfection,mRNA and protein level of target gene survivin was detected by Real -time PCR and Western blot.The ability of miR - 203 was confirmed with dual - luciferase activity assay.Cell growth,proliferation apoptosis and sensitivity to TRAIL and celecoxib were determined by growth curve,MTT assay and flow cytometry.Results Predicting outcomes of TargetScan indicated that survivin was target of miR - 203.After over - expression of miR - 203,Real - time PCR and Western blot showed that mRNA and protein level of survivin were down - regulated,respectively ( P ＜ 0.05 ). Dual - luciferase activity assay demonstrated that survivin was direct target of miR -203.Growth curve and MTT assay revealed that growth and proliferation ability of miR - 203 group were significantly suppressed ( P ＜ 0.05 ),and sensitivity to TRAIL and celecoxib was increased.U251cells transfected miR -203 had a higher proportion of apoptosis than control group ( P ＜0.01).Conclution miR- 203 is an anti -proliferation and pro- apoptosismiRNA which can specifically inhibit survivin expression.In the future,miR -203 may be an attractive target for therapeutic intervention in glioma. Key words: MicroRNA - 203; Glioma; Survivin; Cell growth; Cell proliferation