Influence of Microwave Frequency and Gas Humidity on the In-Vitro Blood Coagulation in Cold Atmospheric Pressure Plasma

Jie Yu,Kama Huang,Li Wu

doi:10.3390/pr9101837

Abstract

In this article, the effects of microwave frequency (2450 MHz and 5800 MHz) and gas humidity (1%, 2%, 3%, 4%, 6% and 8%) on in vitro blood coagulation with cold atmospheric pressure plasma (CAPP) were investigated. The generation of reactive oxygen species (ROS, OH, O) was measured by optical emission spectra. The exposure temperature on blood droplets under treatment was below 55 °C in all cases, to avoid the thermal effect of plasma on the blood clotting. Investigations showed that, with the increase of frequency, the doses of ROS increased, the blood sample presented a more serious collapse and its surface became drier. The humidity of ionized gas can also accelerate the generation of ROS and the process of blood clotting. Our results propose a method to accelerate in vitro blood coagulation in CAPP by adjusting microwave frequency and gas humidity, and suggest a clinical benefit for plasma treatment as a coagulation device in surgery.

Highlights

Plasma medicine has emerged as an independent discipline [1,2,3], which has brought about tremendous changes to the medical domain
Reactive atomic oxygen (RAO) and other likely reactive oxygen species (ROS) on the activation of erythrocyte–platelet particles have been considered as a plausible mechanism of blood coagulation [10,11,12]
This paper investigated the effects of microwave frequency (2450 MHz and 5800 MHz) and different ratios of H2 O/Ar mixed gas (1%, 2%, 3%, 4%, 6% and 8%) on the ROS and in vitro blood coagulation with a coaxial plasma source

Summary

Introduction

Plasma medicine has emerged as an independent discipline [1,2,3], which has brought about tremendous changes to the medical domain. Yan et al [9] described a pulsed cold plasma-induced blood coagulation via direct contact In these plasma generators the reactive oxygen species (ROS) have played a key role in biological applications. This paper investigated the effects of microwave frequency (2450 MHz and 5800 MHz) and different ratios of H2 O/Ar mixed gas (1%, 2%, 3%, 4%, 6% and 8%) on the ROS and in vitro blood coagulation with a coaxial plasma source. MHz) and different ratios of H2O/Ar mixed gas (1%, 2%, 3%, 4%, 6% and 8%) on the2ROS and in vitro blood coagulation with a coaxial plasma source. The matching load is connected to the circulator port 3 for absorbing reflected power.The. The wave-to-coax wave-to-coaxconverter converterisisisolated isolatedtotothe theother otherport portofofaadual-directional dual-directionalcoupler couplertotocouple couple the microwave power to the coaxial structure.

Methods

Omixture

Effect of Microwave Frequencies and Gas Humidies on Blood Coagulation

Conclusions