Influence of microRNA-28-3p on biological behariors of giant cell tumors of bone

Abstract

Objective To determine the expression of microRNA (miRNA, miR)-28-3p in giant cell tumor of bone and its effect on the neoplasm biological behaviors. Methods The samples including tumor and adjacent normal tissues from 20 patients with giant cell tumor of bone were harvested. Real-time quantitative polymerase chain reaction (Real-time PCR) with Taqman probe technique and fluorescence in situ hybridization were applied to ascertain miR-28-3p expression in tumor tissue and adjacent normal tissues. Primary bone giant cell tumor stromal cells (GCTSCs) were used as target cells were transfected with miR-28-3p bionic agent and inhibitor, and microscopic observation and methyl thiazol tetrazolium (MTT) test were applied to evaluate the influence of miR-28-3p on proliferative activity of GCTSCs. Results The real-time PCR tests showed that miR-28-3p expression in tumor tissue was significantly lower than that in peri-tumorous normal tissues (0.98±0.11 vs. 3.27±0.65, P=0.003). Fluorescence in situ hybridization showed lower fluorescence value inside tumor than that in adjacent normal tissues. After transfection of GCTSCs with miR-28 agomir, the cell proliferation activity and the cell counts were significantly reduced as compared with those in control group (0.63±0.12 vs. 1.02±0.14, P=0.024). On the contrary, after transfection GCTSCs with miR-28 antagomir, the cell proliferation activity and the cell counts were significantly increased as compared with those in control group (1.73±0.14 vs. 0.97±0.14, P=0.002). Conclusion MiR-28-3p is down-regulated in bone giant cell tumor, suggesting its antitumor effect. Key words: MicroRNA-28-3p; Giant cell tumor of bone; Gene expression