INFLUENCE OF MICROBIAL FERMENT ON PLASMA D-LACTIC INDUCED BY METHYLPREDNISOLONE

Abstract

A260 Aims: . To investigate the changes of intestinal barrier permeability from methylprednisolone, and the influence of microbial ferment on such changes, so as to provide new testimony for further prevent early bacterial infection after transplantation. Methods: 70 healthy male Wistar rats were divided into three groups: control (N)? immunosupressant(MeP) and microbial ferment(MiF) groups, each group was further divided into four subgroups :0, 3, 5, 7day, at each time point the rats were killed to get blood and some tissue by sterile, and to assay plasma D-lactic and gut microbial ecology. Results: Compared with N groups plasma D-lactic in Mep groups increased significantly at 3, 5, 7day subgroups(0N 0.30±0.03, 3N 0.31±0.03 vs 3MeP 0.89±0.30, p<0.01; 0N 0.30±0.03, 5N 0.32±0.02 vs 5MeP 0.96±0.40, p<0.01; 0N 0.30±0.03, 7N 0.31±0.03 vs 7MeP 0.86±0.40, p<0.05); Gut microbial ecology did not change any. Compared with Mep groups, D-lactic in 7 day MiF sugroup decreased obviously(7MeP0.86±0.40 vs 7MiF0.27±0.10, p<0.01), yet there were no difference at 3, 5day between MeP and MiF groups; Bifidobacterium(TPY) and yeast(MRS) in MiF groups increased obviously at 5, 7day than that in MeP groups(5MiF MRS 8.69±0.28 vs 5MeP MRS8.27±0.35, p<0.05; 7Mif TPY 9.23±0.66 vs 7MeP TPY 8.31±0.49, p<0.05; 7MiF MRS 9.29±0.55 vs 7MeP MRS8.23±0.54, p<0.01), yet there was no difference between the 3 day groups. Conclusions: Methylprednisolone can result in intestinal barrier permeability increase, and microbial ferment can decrease the permeability from methylprednisolone.