Abstract

Artificial insemination has been widely known among breeders as an effective reproductive technology by inserting sperm collected from male animals into the reproductive tract of female animals for the purpose of improving genetic quality. The success of AI is influenced by several factors, namely semen quality, inseminator expertise, livestock health, and other supporting factors. Good semen quality is one of the determining factors for the success of artificial insemination. One of the quality standards for frozen semen according to SNI is the level of motility post thawing minimum 40%. Motility level post thawing very dependent on technique thawing used. Thawing is the process of re-thawing frozen semen at physiological body temperature to reactivate sperm cells. Thawing can have an impact on reducing the quality of spermatozoa if not done properly. Temperature and time accuracy thawing will maintain sperm viability and motility, so that sperm quality is maintained and the success rate of artificial insemination will be high.

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