Influence of metabolic inhibitors on the capacity of spleen cells to form hemolytic plaques and rosettes.

Abstract

SummaryTreatment of normal peritoneal exudate cells with antisheep red blood cell antibodies passively immunized some of the cells (presumably macrophages), i.e., conferred on them the capacity to form hemolytic plaques and rosettes. Preincubation with puromycin inhibited plaque and rosette formation by immune spleen cells of actively immunized animals but not by passively immunized peritoneal exucate cells. Puromycin inhibited protein synthesis in both cell populations equally strongly. Sodium fluoride depressed plaque formation by immune spleen cells of actively immunized animals, but not by passively immunized cells. Sodium fluoride treatment had no effect on rosette formation by either actively or passively immunized cells.