Influence of mesh size and core penetration on estimates of deep-sea nematode abundance, biomass, and diversity

Abstract

It is essential to maximize the information that can be gathered in deep-sea studies by thoroughly assessing sample processing methods. Nematodes are commonly used for the study and monitoring of deep-sea floor habitats, but the potential effects of different methods on the quantification of community attributes remain to be quantified. Here, we consider key methodological elements by comparing the effect of sediment depth and mesh size (63, 45, and 32 μm) on: (1) estimates of nematode community attributes, and (2) the sampling effort required to detect changes in these attributes at a bathyal site on the Chatham Rise, south-west Pacific Ocean. The 63 μm mesh retained most (95%) of the nematode biomass but a lower proportion (53–71%) of the nematode abundance. Retention efficiency of common species on this mesh ranged from 12 ( Hapalomus sp.) to >88% ( Comesomatidae spp.). The 63 μm mesh yielded significantly lower diversity estimates than the finer meshes, and failed to detect differences in community structure observed using the 45 and 32 μm mesh sizes. Sediment depth had a substantial effect on all measured community attributes, highlighting the importance of sufficient core penetration into the sediment (≥5 cm) for adequately characterizing nematode distribution. Power analysis showed that using a 32 μm mesh and deepest core penetration led to relatively few (3–8) samples being required to detect significant changes in nematode diversity indices relative to coarser mesh sizes. Characterization of nematode diversity and community structure using appropriate and robust methods of sampling is suggested as a sensitive and efficient tool for the assessment of anthropogenic impacts on deep-sea ecosystems.