Influence of Mesenchymal Stem Cells on Qualitative and Quantitative Characteristics of Energy Transformation in Muscle Tissue of Lower Extremity in Ischemia.

Abstract

Energy transformation required for the performance of multiple energy dependent functions of the body, i.e. mechanical, chemical, and electrical, is important part of cell activity. Energy dependent functions become slower at intracellular level due to decreased ATP by 10–15% and stops completely with 25–30% decrease in ATP in the cell. Adenylic nucleotide concentration measures the mismatch between the energy demand and energy production in the cell's mitochondrial oxidative phosphorylation.A study was done with pubescent Wistar rats with body mass of 160–200 grams. The ischemia of the lower extremities was achieved by ligation of femoral artery. Bone marrow was taken from femoral, humoral and tibial bones. Cell culture was grown in Eagle medium IGLA MEM, enriched with L‐glutamine, 10% calf embryo serum with antibiotics added and 5 million of cell were injected intramuscularly in the experimental animals. The concentration of ATP, ADP and AMP in the gastrocnemius muscle measured by means of thin‐layer filtration on silica gel plates on 3rd, 5th, 7th, 14th, 21st and 28th day after injection of mesenchymal stem cells (MSC).The concentration of ATP decreased significantly in the control group after ischemia in consecutive study on specified days. It has been proved that MSC ejection redistributes the pool of adenylic nucleotides towards the increased ATP biosynthesis, so that such parameters of energy metabolism as energy charge, energy potential, thermodynamic control of respiration are increased and the correlation between the components of adenylate kinase reaction is decreased.This study showed that the ability of MSC provide protective action on energy homeostasis by means of the synthesis of large amount of cytokines and growth factors which stimulate the synthesis of glycolytic and Krebs cycle enzymes, increase glucose utilisation and ATP synthesis. Thus, activation of ATP synthesis restores the level of ATP and high energy phosphate bonds.This abstract is from the Experimental Biology 2018 Meeting. There is no full text article associated with this abstract published in The FASEB Journal.