Abstract

In cellular membranes, proteins and lipids are in sensitive macromolecular interaction influencing each other. To evaluate this interaction, the multi-drug transporter LmrA from Lactococcus lactis was functionally reconstituted in vesicles consisting of 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC), 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC), DMPC+10 mol% cholesterol and the model raft mixture DOPC/1,2-dipalmitoyl-sn-glycero-3-phosphocholine/cholesterol (1:2:1) and in natural membrane lipids at 30 °C. The lateral structure and organization of these proteoliposomes were modulated using high hydrostatic pressure. A sharp pressure-induced fluid-to-gel phase transition is observed without an extended two-phase region. The possibility for lipid sorting, such as for DMPC/cholesterol bilayers, has an inhibitory effect on the LmrA activity. A fluid-like membrane phase over the whole pressure range with suitable hydrophobic matching, such as for DOPC, prevents the membrane protein from high-pressure inactivation up to 200 MPa. Under high-pressure conditions, highest LmrA activities, exceeding those at ambient pressure, are achieved for heterogeneous lipid matrices with a small hydrophobic mismatch and the ability of lipid sorting.

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