Influence of medium composition on penicillin V production in a stirred tank reactor

Abstract

Penicillin production with a high-producing strain Penicillium chrysogenum was investigated under well-controlled conditions in a stirred tank reactor with complex media containing lard oil and lactose on the one hand, and lactose on the other hand. With lard oil, cell growth and product formation rates were higher, and the production time was shorter by 40 h than without lard oil. On account of the longer production time without lard oil, the amount of β-lactam compounds was higher (29.93 g 1 −1), but the mole fraction of the decomposed products (penicilloic acid and penilloic acid) was larger (0.282) than the amount of penicillin V (23.25 g 1 −1 and the decomposed mole fraction (0.0747) with lard oil. The final product concentrations were about the same (20.86 g 1 −1 or 35 462 IU ml −1 with lard oil, and 20.43 g 1 −1 or 34510 IU ml −1 without lard oil). The mole fractions of the by-product (p-OH-penicillin V) were 0.0365 and 0.066. The substitution of lard oil with lactose is possible without a considerable reduction of process performance.