Influence of Maternal High Salt Diet on Systemic and Renal Renin of Newborn and Adult Male Offspring Wistar Rats

Abstract

Objective to evaluate the renal and circulating renin of mothers whose received high sodium diet during pregnancy and their male offspring at birth and adult life. Methods and Results (mean±SEM p<0.05 n=8/group): female Wistar rats received normal salt diet (NS 1.3%) or high salt diet (HS 8.0% NaCl) only during pregnancy (dams). The male adult offsprings (offNS, offHS), were fed NS since birth until 12-week- age. The litter weight, weight gain and blood pressure of offsprings was not different between groups. The plasma renin activity (PRA- ng/mL/h) was lower in dams HS (0.67±0.5) than NS (2.65±0.2). PRA was not different among newborn groups however, PRA was lower in adult offHS (2.2±0.6) than offNS (4.2±0.4). The renal renin granules (%) were lower in HS (29.7±3) than in NS (50±2) dams, and in adult offHS (38.4±4.2) than offNS (60.7±5.0). The renal renin gene expression (IDVrenin/β-actin) was not different in dams (cortex: NS 0.50±0.06, HS 0.43±0.01; medulla: NS 0.41±0.01, HS 0.39±0.01) and newborn (NS 1.60±0.03; HS 1.62±0.01), but it was lower in renal cortex (1.0±0.1) and medulla (0.6±0.06) of adult offHS than offNS (1.5±0.2; 1.5±0.2 respectively). Conclusion Decreased renin levels observed in adult offspring was transmitted from mother during pregnancy. Financial support: CAPES and FAPESP