Influence of Marmix premix on the state of lipid peroxidation and indices of non-specific resistance of the organism of pregnant mares with microelementosis

A R Shcherbatyy,V L Fedorovych,B V Gutyj,B O Lukashchuk,L G Slivinska

doi:10.15421/021914

Abstract

We observed the processes of lipid peroxidation, characterized by increased content of intermediate and its end products (diene conjugates, lipid hydroperoxides, malonic dialdehyde), in the blood of the pregnant mares. The changes of the data of indicators in the organism of the mares at the 9–11 months of pregnancy were established. The level of diene conjugates in the blood of animals was 3.6 ± 0.12 μmol/l, 33.3 and 44.0%, which was higher compared to non-pregnant mares and mares at 4th month of pregnancy. The concentration of lipid hydroperoxides in the blood of mares increased by 100% and by 42.9% in the blood of non-pregnant mares and mares at 4th month of pregnancy. The concentration of malondialdehyde in the blood of mares increased by 75.0%, 51.2% and 25.0% compared to non-pregnant mares and mares at 4th and 7th month of pregnancy, respectively. The results of our research showed that the mineral-vitamin premix Marmix had a positive influence on the state of lipid peroxidation products, contributed to their lowering in the blood of pregnant mares, which demonstrated antioxidant action and stimulated the function of the immune system. It was established that the bactericidal and lysozyme activity of the blood serum and the concentration of circulating immune complexes increases in the mares with lack of trace elements. It was shown that feeding mares during the 9–11 months of pregnancy with the mineral-vitamin premix Marmix had a positive influence on the state of humoral factors of nonspecific resistance. In particular, the concentration of bactericidal activity of serum increased by 31.0%, lysozyme activity of blood serum – 45.4% and contributed to the decrease of the concentration of circulating immune complexes in serum by 3.8 times. The use of mineral-vitamin premix Marmix during 60 days in feeding pregnant mares caused a restoration of the clinical status, a decrease in the content of lipid peroxidation products.

Highlights

There is a significant number of reports concerning the role of lipid peroxidation in the development of many diseases of noninfectious and infectious etiology (Wingfield, 1984; Clarke et al, 1987; Holovakha et al, 2018)
The following conclusions can be drawn according to the obtained results: the mineral-vitamin premix Marmix inhibits the processes of lipid peroxidation, namely, reduces the concentration of oxidation products, thereby reducing the toxic influence on the cells of the body, which contributes to its recovery and the disappearance of symptoms of microelementosis, anemia, disturbance of liver and kidney function
Mares, during the period of pregnancy, are very sensitive to malnutrition and conditions of retention. This especially applies to the mares during the last months of the pregnancy, which are characterized by intensive use of the mineral, energetic and plastic resources of the mother’s body in the formation of fetal tissues (Shcherbatyy et al, 2017)

Summary

Introduction

There is a significant number of reports concerning the role of lipid peroxidation in the development of many diseases of noninfectious and infectious etiology (encephalomalation and exudative diathesis, proteinuria, gastrointestinal, respiratory and metabolic diseases, in particular, microelementosis, kidney damage, liver, endometritis, mastitis, etc.) (Wingfield, 1984; Clarke et al, 1987; Holovakha et al, 2018). The research objective was to determine effectiveness of the mineral-vitamin premix Marmix in the system of complex measures for prevention of microelementosis, to analyze its influence on the state of peroxidation of lipids system and non-specific resistance of the organism of pregnant mares.

Results

Conclusion