Influence of lornoxicam and triamcinolone on the dynamics of eye remodeling in concanavalin model of inflammation

Abstract

AbstractPurpose To compare the effects of NSAID lornoxicam and corticosteroid triamcinolone on the dynamics of eye remodeling in concanavalin model of inflammation.Methods Eye of Wistar rat was injected with 0,5 mcg (2,5 mcl) concanavalin A (ConA) in vitreous cavity. After 20 min rats received a saline, 16 mcg lornoxicam or 80 mcg triamcinolone intravitreal injection (2 mcl). On 1 and 2 day after that the medication was administered systemically. Histological evaluation of retinas was made on 7, 14, 28 and 56 days after inravitreal injection of ConA.Results The total frequency of cararactogenesis after the injection of ConA was 72%. Lornoxicam and triamcinolone suppressed the development of cataracts. Lornoxicam 6 times decreased the total frequency of eye hemorrhage. After 7 days following ConA injection lornoxicam decreased thickness of more retina layers than triamcinolone. After 14 days triamcinolone increased the thickness of the outer plexiform, inner nuclear, inner plexiform layers as compared with the control group. At day 14 lornoxicam but not triamcinolone reduced the thickness of ganglion cell layer (GCL). At day 28 both drugs reduced the thickness of GCL. Phospholipase A2 and cyclooxygenase (COX) blocking reduced structural abnormalities of the retina, but its thickness increased on 56 day after triamcinolone administration.Conclusion Lornoxicam prevented the development of proliferative vitreoretinopathy (PVR), reduced the severity of symptoms typical for proliferative diseases of the eye. It also inhibited the proliferation of retinal cells. Triamcinolone impaired the overall health of animals, less prevented the development of PVR compared to lornoxicam. Its effect on the proliferation of retinal cells was ambiguous.