Abstract
The article contains the experimental studies of the liposomal drug based on plant raw materials ― hypericum (Hypericum perforatum L.) effect on the intensity of oxidative modification of proteins (OMP) in the blood and milk of cows with subclinical mastitis. Studies have shown that cows with signs of subclinical form of mastitis in the serum have an increase in the content of aldehyde-derived OMP370 and ketone-derived OMP430, respectively, 1.3 and 1.2 times relative to similar indicators in healthy animals. In the milk of sick cows, the content of derivatives OMP370 and OMP430 was1.99 and 2.29 times higher, respectively, than in animals of the control group. At the beginning of the study sick cows’ milk was recorded a significantly low value of the activity of the enzymatic link of antioxidant protection ― superoxide dismutase. At the same time, a 2.6-fold (P<0.001) increase in the number of somatic cells was noted compared to their number in the milk of clinically healthy cows. Intracisternal injection of liposomal drug to cows caused a decrease in the intensity of oxidative processes. In the blood of sick cows the content of aldehyde derivatives OMP370 on the 9th day of the experiment was 23.1% (P<0.05) less than before the drug, and in milk the content of OMP370 decreased by 61.8% (P<0.01). Similar changes were observed with respect to the level of ketone derivatives. In particular, on the 9th day of the experiment, the content of OMP430 decreased by 11.7% (P<0.05) compared with its value in the blood of sick animals before the introduction of the study drug, and in milk it decreased by 64.2% (P<0.01). During the treatment on the 9th day of the experiment, the number of somatic cells in milk decreased by 41.8% (P<0.01). In the course of treatment on the 3rd and 9th day there was a tendency to increase superoxide dismutase activity in the milk of sick cows compared with the beginning of the experiment. Thus, intracisternal injection of liposomal drug to cows with subclinical mastitis leads to a decrease in aldehyde and ketone derivatives of proteins oxidative modification in serum and milk. At the same time, an increase in the activity of the enzymatic link of antioxidant protection and a decrease in the number of somatic cells in the milk of cows were recorded.
Highlights
The problem of cattle mastitis in Ukraine is defined by researchers as the main issue of the livestock industry
Studies have shown that cows with signs of subclinical form of mastitis in the serum have an increase in the content of aldehyde-derived OMP370 and ketone-derived OMP430, respectively, 1.3 and 1.2 times relative to similar indicators in healthy animals
The introduction of the studied liposomal drug caused a decrease in the intensity of oxidative processes, as indicated by a decrease of 23.1% (P
Summary
The problem of cattle mastitis in Ukraine is defined by researchers as the main issue of the livestock industry. Any pathological processes in the body are accompanied by the activation of free radical processes in the tissues and organs of a sick animal. All functionally important free radicals that are formed in the body contain oxygen. These compounds are combined by the term “reactive oxygen species” (ROS). The main forms of ROS generated in a living organism are: superoxide radical, hydroxyl radical, nitric oxide, peroxyl radical, hydrogen peroxide and others. The main forms of ROS are primarily normal components of cellular metabolism and perform certain biological functions. Their reactive aggressiveness is restrained by a powerful antioxidant system. With the development of pathological processes, this balance is disturbed in the direction of uncontrolled synthesis of ROS, which ends with the formation of oxidative stress
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