Influence of Light of Different Spectral Compositions on the Growth, Photosynthesis, and Expression of Light-Dependent Genes of Scots Pine Seedlings.

Pavel Pashkovskiy,Yury Ivanov,Alexander Shmarev,Vladimir V Kuznetsov,Alexandra Ivanova,Vladimir D Kreslavski,Suleyman I Allakhverdiev,Valeriya Strokina,Alexander Kartashov

doi:10.3390/cells10123284

Pavel Pashkovskiy, Yury Ivanov + Show 7 more

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https://doi.org/10.3390/cells10123284

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Abstract

Varying the spectral composition of light is one of the ways to accelerate the growth of conifers under artificial conditions for the development of technologies and to obtain sustainable seedlings required to preserve the existing areas of forests. We studied the influence of light of different quality on the growth, gas exchange, fluorescence indices of Chl a, and expression of key light-dependent genes of Pinus sylvestris L. seedlings. It was shown that in plants growing under red light (RL), the biomass of needles and root system increased by more than two and three times, respectively, compared with those of the white fluorescent light (WFL) control. At the same time, the rates of photosynthesis and respiration in RL and blue light (BL) plants were lower than those of blue red light (BRL) plants, and the difference between the rates of photosynthesis and respiration, which characterizes the carbon balance, was maximum under RL. RL influenced the number of xylem cells, activated the expression of genes involved in the transduction of cytokinin (Histidine-containing phosphotransfer 1, HPT1, Type-A Response Regulators, RR-A) and auxin (Auxin-induced protein 1, Aux/IAA) signals, and reduced the expression of the gene encoding the transcription factor phytochrome-interacting factor 3 (PIF3). It was suggested that RL-induced activation of key genes of cytokinin and auxin signaling might indicate a phytochrome-dependent change in cytokinins and auxins activity.

Highlights

The quality of light is an important factor in regulating plant growth and development during ontogenesis, including germination, photomorphogenesis, flowering induction, etc
We studied the transcription of genes responsible for light signaling and the synthesis of secondary metabolites, such as the transcription factors phytochrome-interacting factor 3 (PIF3), CHS, and STS
We tried to answer the question of what spectral range of light can be most favorable for the growth of P. sylvestris seedlings

Summary

Introduction

The quality of light is an important factor in regulating plant growth and development during ontogenesis, including germination, photomorphogenesis, flowering induction, etc. Green and far-red light (FRL) predominate under the forest canopy because light in the red and blue ranges of the spectrum is effectively absorbed by the chlorophyll of taller plants. This forces the seedlings of most woody plants to adapt to indifferent light qualities [1]. It was previously shown that blue light (BL), on the contrary, inhibits shoot growth [2,3].

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