Influence of LED light spectra on in vitro somatic embryogenesis and LC-MS analysis of chlorogenic acid and rutin in Peucedanum japonicum Thunb.: a medicinal herb.

Chia-Chen Chen,Chao-Lin Kuo,Hsin-Sheng Tsay,Dinesh Chandra Agrawal,Hung-Chi Chang,Ren-Jye Lee,Chi-Rei Wu,Maw-Rong Lee

doi:10.1186/s40529-016-0124-z

Abstract

Background Peucedanum japonicum Thunb, an important medicinal herb is reported to possess pharmacological properties such as anti-obesity, anti-oxidant, anti-inflammatory, anti-bacterial, anti-diabetic and anti-platelet aggregation. The present study aimed to develop an in vitro plant regeneration system of P. japonicum via somatic embryogenesis and to analyse chlorogenic acid and rutin contents in a few commercially available plant products of P. japonicum in Japan and Taiwan markets, and tissue culture plants derived from somatic embryos.ResultsInduction of somatic embryogenesis could be achieved when root derived calli after three subcultures were transferred from Murashige Skoog’s salts and vitamins (MS basal) medium with 2,4-dichlorophenoxyacetic acid (2,4-D) (0.1–5 mg/L) to a medium with abscisic acid (ABA) (0.5–4 mg/L), or exposed to eight different light spectra provided by light-emitting diode (LED) sources. Concentrations of ABA and LED light spectra had an influence on number of somatic embryos induced and proliferation of callus. Development of secondary somatic embryos and conversion of embryos to plantlets was achieved on a medium with ABA, or their exposure to red or blue lights in a special incubation chamber. Four months old tissue culture plants derived from somatic embryos showed significantly higher levels of chlorogenic acid (10.5 mg/g dw) compared to commercial product sold in Japanese market (0.55 mg/g dw). However, rutin was absent in tissue culture plants in contrast to commercial sample (0.33 mg/g dw).ConclusionIn this report, we describe in vitro plant regeneration system in P. japonicum via somatic embryogenesis and production of chlorogenic acid in tissue culture plants. The present study has application in further tissue culture propagation of elite plant material with high chlorogenic acid content, and identification of high yielding plants with the LC–MS method.

Highlights

Peucedanum japonicum Thunb, an important medicinal herb is reported to possess pharmacologi‐ cal properties such as anti-obesity, anti-oxidant, anti-inflammatory, anti-bacterial, anti-diabetic and anti-platelet aggregation
The present study was carried out to develop a plant regeneration system of P. japonicum via somatic embryogenesis and to carry out LC–MS analysis of chlorogenic acid and rutin contents in a few commercial products of the species marketed in Japan and Taiwan, and tissue culture plants derived from somatic embryos
The maximum chlorogenic acid content (10.5 mg/g dw) was recorded in tissue culture plants derived from somatic embryos

Summary

Introduction

Peucedanum japonicum Thunb, an important medicinal herb is reported to possess pharmacologi‐ cal properties such as anti-obesity, anti-oxidant, anti-inflammatory, anti-bacterial, anti-diabetic and anti-platelet aggregation. While studying the radical scavenging activity of P. japonicum, (Hisamoto et al 2003) isolated 17 compounds from the leaves, of which several compounds played an important role in the potent antioxidant activity. Nugara et al (2014) demonstrated that partially purified hexane phase (HP) had a crucial role in regulating lipid metabolism-related gene expression and energy expenditure in vitro, inferring that alterations in lipid metabolism rather than inhibition of lipid absorption may be the primary mechanism for the antiobesity activity of P. japonicum. P. japonicum could prevent the obesity via the up-regulation of FXRα that plays a pivotal role in the obesity related lipid metabolism (Nukitrangsan et al 2011, 2012)

Methods

Results

Conclusion