INFLUENCE OF LEAD ACETATE IN CONJUNCTION WITH STEARATES ON THE BONE BRAIN AND PERIPHERAL BLOOD OF SUSPICIOUS ANIMALS

Abstract

In modern conditions, industrial pollution of the environment with lead is quite significant and has an adverse effect on the body. It has pronounced cumulative properties and is accumulated in bones in the form of insoluble tri-phosphorus. However, under the influence of certain conditions, its reserves in the bones become mobile; it transits into the bloodstream and can cause poisoning, even in the sharp form.
 The aim – to study the effect of lead acetate in the body at sub-toxic doses on the background of drinking water with stearates content.
 Materials and Methods. To study the influence of low-frequency lead in isolation and in combination with stearates under conditions of a subacute sanitary-toxicological experiment, the animals were divided into four groups of 7 animals each. The group 1 of animals was a control group. The group 2 of animals consumed dechlorinated water from the city water supply (water + AcPb). Animals of the groups 3 and 4 also consumed dechlorinated water from the city water supply, but with an admixture of sodium stearate (group 3 StNa + AcPb) and potassium stearate (group 4 StK + AcPb) at a dose of 1/250 LD50. After a 30-day application of these waters to animals, the groups 2, 3 and 4 were orally administered lead acetate at a dose of 7 mg/100 g of body weight (1/110 LD50).
 Results and Discussion. As a result of the action of lead acetate in a dose of 70 mg/kg and stearates, there was an increase in the bone marrow of the number of promyelocytes, stem cells, segmental neutrophils of lymphocytes and normocytes. However, the number of myelocytes and metamyelocytes decreased. Lead acetate and stearates caused an increase in the number of rodenuclear neutrophils, eosinophils, monocytes, lymphocytes and a decrease in the number of segmental neutrophils in the blood of experimental animals. Unlike intact animals in the groups 2, 3 and 4, there were observed events of functional failure of the erythrocytes system such as anisocytosis, poikilocytosis and hypochromia.
 Conclusions. 1. Thus, with the action of lead acetate at a dose of 70 mg/kg in combination with sodium and potassium stearates, there was an increase in the bone marrow of promyelocytes, stabnoid, segmented neutrophils of lymphocytes and normocytes. The number of myelocytes and metamyelocytes decreased.
 
 Lead acetate in combination with sodium and potassium stearates caused an increase in the number of stab neutrophils, eosinophils, monocytes, lymphocytes and a decrease in the number of segmented neutrophils in the blood of experimental animals.