Influence of lactate accumulation on Na +,K +-ATPase activity of ischemic and post-ischemic brain

Abstract

In this study the cerebral Na +,K +-ATPase activity as well as selected parameters of oxidative metabolism and electrophysiological function were assessed in normoglycemic and hyperglycemic rats which were exposed to ischemia produced by eletrocautery of the vertebral arteries and reversible occlusion of the carotid arteries. In hyperglycemic animals 0.5 h of ischemia was associated with massive accumulation of lactate (34 μmol·g −1) and enhanced Na +,K +-ATPase activity (116% control), whereas normoglycemic animals showed more moderate lactate accumulation (17 μmol·g −1) and normal Na +,K +-ATPase activity (102% control). In normoglycemic animals release of the carotid clamps and recirculation for 0.5-1.5 h was associated with a normalization of the lactate levels and a decrease in Na +,K +-ATPase activity (68–72% control). Restituted hyperglycemic animals showed metabolic changes which seemed related to the blood pressure, with hypotensive hyperglycemic animals showing continuing massive lactacidosis (30–35 μmol·g −) and enhanced Na +,K +-ATPase activity (108–110% control), whereas normotensive hyperglycemic animals showed progressive decreases in lactate level (14–20 μmol·g −1) and normal or mildly suppressed Na +,K +-ATPase activity (88–97% control). These patterns of change suggest that the reperfusion of the post-ischemic hyperglycemic-hyperlactacidotic brain was inadequate or non-homogenous.