Influence of L-tryptophan and auxins applied to the rhizosphere on the vegetative growth of Zea mays L.

Abstract

Glasshouse experiments were conducted to evaluate the influence of L-TRP in comparison with indole-3-acetamide (IAM), tryptophol (TOL) and indole-3-acetic acid (IAA) on the growth of Zea mays L. var. Early Sunglow. L-TRP (25 to 2.5×10−5 mg kg−1 soil), IAM (22 to 2.2×10−5 mg kg−1 soil), TOL (20 to 2.0×10−5 mg kg−1 soil), and IAA (22 to 2.2×10−5 mg kg−1 soil) were applied as a soil drench to established uniform seedlings. All treatments were applied in a completely randomized design with 10 replicates. IAM had no significant effect on the plant growth parameters. Shoot height, uppermost leaf collar base distance, internodal distance, and shoot dry and fresh weights were significantly improved upon the addition of TOL (2.0×10−2 mg kg−1 soil), however, the highest concentration (20 mg kg−1 soil) caused a 14.6% reduction in leaf width. L-TRP (2.5×10−3 mg kg-1 soil) also had a significant influence on shoot height, uppermost leaf collar base distance, internodal distance and fresh weight of shoot compared with the control. The highest concentration of L-TRP (25=mg kg−1 soil) had a negative effect on leaf width and dry weight of the shoot. The most pronounced response on the corn growth parameters was observed with the application of IAA at lower concentrations (2.2×10−5 to 2.2×10−2 mg kg−1 soil) specifically improving root growth. The highest concentration (22 mg kg−1 soil) of IAA had a significant negative effect on plant height, leaf width, stem diameter, shoot fresh and dry weight. These findings indicate that L-TRP applied at the appropriate concentrations can have positive effects on corn growth comparable to pure auxins (TOL and IAA).