Influence of ischemic post-conditioning on the lipid peroxidation after lung transplantation in canis

Abstract

Objective Ischemia-reperfusion injury may occur in the donor lung during lung transplantation. As a response to ischemia-reperfusion injury, ischemic post-conditioning provides an intrinsic protection. The study was aimed at identifying the influ-ence of ischemic post-conditioning on the function of donor lung in dogs by measuring the changes of lipid peroxidation during early reperfusion. Methods Twelve orthotopic left lung transplantations were performed by using 24 paired dogs. The animals were divided randomly into the ischemic post-conditioning group and the control group. For dogs (6 pairs) in the control group, no intervention of ischemic post-conditioning was performed during routine left lung transplantation, for the rest dogs in the ischemic post-conditioning group, 3 cycles of iscbemic post-conditioning with reperfusion(for 10 seconds)-interruption (for another 10 seconds) were performed after implantation of donor lungs obtained routinely. Content of malonaldehyde(MDA) and activity of superoxide dismutase (SOD) in the specimens of donor lungs were measured with Commassie Brilliant Blue 1 and 2 hours after transplantation, respectively. Mean-while, fresh pulmonary specimens obtained at different time points were stained with hematoxylin and eosin(HE). A statistical software SPSS 13.0 was used in the analysis. A significant difference was indicated by P <0.05. Results Transplantations were completed in 12 receiver canis, the mean time for anastomoses was(35.92 ± 1.73)min. The contents of MDA in the experimental group was sig-nificantly lower than that of control gronp(P <0.05)1 and 2 hours after transplantation. The activity of SOD was significantly higher in the experimental group than that of control group 1 and 2 hours after transplantation(P < 0.05). Under the light microscopic exami-nation, the inflammatory changes observed were comparative mild in the ischemic post-conditioning group. Conclusion The accumu-lation of reactive oxygen species induced by canine lung transplantation may be decreased with iachemic post-conditioning, an interven-tion that attenuates damage to the donor lungs and improves their function during ischemia reperfusion. Key words: Lung transplantation; Ischemic post-conditioning; Ischemic reperfusion injury; Lipid perioxidation