Influence of iridoid glycoside containing host plants on midgut β-glucosidase activity in a polyphagous caterpillar, Spilosoma virginica Fabricius (Arctiidae)

Abstract

Iridoid glycosides are secondary plant compounds that have deterrent, growth reducing or even toxic effects on non-adapted herbivorous insects. To investigate the effects of iridoid glycoside containing plants on the digestive metabolism of a generalist herbivore, larvae of Spilosoma virginica (Lepidoptera: Arctiidae) were reared on three plant species that differ in their secondary plant chemistry: Taraxacum officinale (no iridoid glycosides), Plantago major (low iridoid glycoside content), and P. lanceolata (high iridoid glycoside content). Midguts of fifth instar larvae were assayed for the activity and kinetic properties of β-glucosidase using different substrates. Compared to the larvae on T. officinale, the β-glucosidase activity of larvae feeding on P. lanceolata was significantly lower measured with 4-nitrophenyl-β-d-glucopyranoside. Using the iridoid glycoside aucubin as a substrate, we did not find differences in the β-glucosidase activity of the larvae reared on the three plants. Heat inactivation experiments revealed the existence of a heat-labile and a more heat-stable β-glucosidase with similar Michaelis constants for 4-nitrophenyl-β-d-glucopyranoside. We discuss possible mechanisms leading to the observed decrease of β-glucosidase activity for larvae reared on P. lanceolata and its relevance for generalist herbivores in adapting to iridoid glycoside containing plant species and their use as potential host plants.