Influence of inorganic microelements on the production of camptothecin with suspension cultures of Camptotheca acuminata

Abstract

The effects of eight microelements (I−, BO33−, MoO42−, Co2+, Cu2+, Mn2+, Fe2+, Zn2+) on the biosynthesis of camptothecin and the growth of suspension cultures of Camptotheca acuminata were studied. The increase of I− to 25 μM l−1, Cu2+ to 1 μM l−1, Co2+ to 2 μM l−1 and MoO42− to 10 μM l−1 in Murashige and Skoog (MS) medium resulted in 1.66, 2.84, 2.53 and 2.04 times higher of camptothecin yield than that in standard MS medium respectively. Combined treatment of I− (25 μM l−1), Cu2+ (1 μM l−1), Co2+ (2 μM l−1) and MoO42− (10 μM l−1) lead to improve cell dry weight, camptothecin content, and camptothecin yield to 30.56 g l−1, 0.0299%, and 9.15 mg l−1, respectively, which were 20.2, 208.9 and 273.8% increment respectively when compared with those of control.