Influence of inoculum variation and nutrient availability on polyhydroxybutyrate production from activated sludge

Abstract

Carbon recovery through polyhydroxybutyrate (PHB) production can create a value-added waste management system. Activated sludge as inoculum enables PHB production using cheap and renewable carbons source, bringing PHB at par to conventional plastics. The PHB accumulating potential of activated sludge needs to be improved to realize the objective. The interaction between the origin of activated sludge, petroleum refinery sludge and brewery sludge, and nitrogen availability was explored to effect culture enrichment, improve PHB accumulation, and polymer characteristics through aerobic dynamic feeding. Consequently, nitrogen excess and limitation enrichment of both sludges produced mix microbial culture with adequate PHB storage of 7.8 ± 0.05%, 14.4 ± 0.04%, 14.4 ± 0.04%, 13.4 ± 0.02% respectively. Batch accumulation revealed higher PHB accumulation of 76.1 ± 0.03% and 71.7 ± 0.05% under nitrogen limitation for PRS and BS enriched under nitrogen excess condition compared to any other combination. The higher decomposition temperature of 285 °C, 293 °C, and a lower melting point of 168 °C, 165 °C with a higher molecular weight of 4.3x105g/mol and semi-crystalline arrangement indicates the potential applications for extracted PHB. PHB production enhanced under nitrogen limited conditions with culture enriched under nitrogen excess condition. However, similar PHB storage, physiochemical property, and overlapping microbial community show an insignificant effect of sludge origin on PHB production.