Influence of induced heme oxygenase-1 on ischemia-reperfusion injury of fatty liver transplantation

Abstract

Objective To investigate whether induced heme oxygenase-1(HO-1)could alleviate the ischemia-reperfusion injury in fatty liver transplantation and the mechanism. Methods Before liver tansplantation, mild steatotic liver was established by high fat diet for 4 weeks, and then the rats were di-vided into 3 groups randomly. In group A(n = 25 ), heroin was injected intraperitoneally 24 h before resec-tion of donor liver. In group B(n = 25 ), heroin was injected intraperitoneally 24 h before resection of donor liver,and rats were infused with ZnPP at the end of surgery after unclamping the vessel. In group C(n = 25),saline was injected intraperitoneally 24 h before resection of donor liver. The liver and serum samples in the recipients were collected 3,6,12,24 and 48 h after transplantation. The activity of HO-1 and TNF-α level were detected. And the histological changes were observed under a light microscope. Results The post-operative activity of HO-1 in group A was significantly higher than that in groups B and C, but there was no significant difference between groups B and C. The TNF-α level in group A was significantly lower than that in groups B and C,but there was no significant difference between groups B and C. All rat livers showed the most serious ischemia-reperfusion injury at 12 h after reperfusion, and the damage was milder in group A than that in the other two groups. Conclusion HO-1 can alleviate the ischemia-reperfnsion in-jury of fatty liver transplantation by inhibiting the TNF-α expression. Key words: Fatty liver; lschemia; Reperfusion injury; Heine oxygenase-1; Liver trans-plantation