Abstract
Effects of incubation temperatures of 21 and 32°C, incubation time, and pH control at 6.0 on alcohol dehydrogenase (E.C.1.1.1.1., alcohol: nicotinanide dinucleotide alcohol dehydrogenase oxidoreductase) activity were evaluated for batch cultures of Streptococcus lactis 60 and C2 in Elliker broth. Without pH control, alcohol dehydrogenase was maximal in both strains after 6h incubation and declined with increasing incubation of stationary phase cells. Increasing growth temperature increased optical density and plate count data at 6h incubation for both strains. Alcohol dehydrogenase activity of 6h cells of Streptococcus lactis 60 was higher with 32°C than with 21°C incubation. For Streptococcus lactis C2, increasing incubation temperature resulted in lower detectable alcohol dehydrogenase activity at 6h incubation. Controlling growth pH at 6.0 increased growth and increased alcohol dehydrogenase activity. Alcohol dehydrogenase activity of Streptococcus lactis grown with pH control did not decline as dramatically during increased incubation time as that of Streptococcus lactis grown without pH control.
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