Influence of incubation temperature on productivity and quality of Spodoptera litura nucleopolyhedrovirus

Abstract

Mass production of baculoviruses by in vivo methods is influenced by several factors like larval age at virus treatment, virus concentration and the incubation temperature. The larval age at virus treatment and virus concentration should be synchronized to result in insect death at a fully grown larval stage to maximize the productivity. Since temperature influences both the growth of the larvae and replication of the virus, we evaluated the influence of incubation temperature on mass production of Spodoptera litura nucleopolyhedrovirus (SpltNPV) at four different temperature regimes viz., 25, 30, and 35 °C and room temperature by diet-surface contamination method. Incubation of early fifth instar larvae dosed with 3932.4 polyhedral inclusion bodies (PIB)/mm 2 at 25 °C enhanced the virus productivity to 6.623 × 10 11 PIB yield/100 inoculated larvae, while it was only 1.779 × 10 11 at 35 °C. The disease progression in the virus treated larvae was slow with median lethal time (LT 50 ) of 208 h at 25 °C as compared to 136 h at 35 °C. In spite of the slow death which means lower production cycles/year, the productivity/year was higher at 25 °C than at other temperatures. The SpltNPV produced at 25 °C was also found to be of superior quality in terms of low bacterial contaminants than at 35 °C. Neonate larval bioassay conducted with viruses produced at different temperature treatments revealed that they were similar in their activity and virulence. Hence our results indicate that maintenance of the SpltNPV production facility at 25 °C would enhance both the virus productivity and quality.