Abstract
Oxidative stress is one of the significant precursors of various metabolic diseases such as diabetes, Parkinson’s disease, cardiovascular diseases, cancer, etc. Various scientific reports have indicated that secondary plant metabolites play an important role in preventing oxidative stress and its harmful effects. In this respect, this study was planned to investigate the phenolic profile and antioxidant and antidiabetic potentials of the aqueous extracts from Turkish Cistus species by employing in vitro methods. In vitro digestion simulation procedure was applied to all extracts to estimate the bioavailability of their phenolic contents. Total phenolic, flavonoid, phenolic acid and proanthocyanidin contents were determined for all phases of digestion. In addition, changes in the quantity of the assigned marker flavonoids (tiliroside, hyperoside and quercitrin) were monitored by High-Performance Thin Layer Chromatography (HPTLC) analysis. The antioxidant activity potentials of the extracts were studied by various methods to reveal their detailed activity profiles. On the other hand, in vitro α-amylase and α-glucosidase enzymes and advanced-glycation end product (AGE) inhibitory activities of the extracts were determined to evaluate the antidiabetic potentials of extracts. The results showed that aqueous extracts obtained from the aerial parts of Turkish Cistus species have rich phenolic contents and potential antioxidant and antidiabetic activities; however, their bioactivity profiles and marker flavonoid concentrations might significantly be affected by human digestion. The results exhibited that total phenolic contents, antioxidant activities and diabetes-related enzyme inhibitions of the bioavailable samples were lower than non-digested samples in all extracts.
Highlights
The Cistaceae family is composed of shrubs, annual or perennial herbaceous plants, and the genus Cistus is one of the broadly distributed members of this family
The phenolic contents of the aqueous extracts were negatively affected by the in vitro human digestion simulation procedure
The aqueous extracts of all Cistus species recorded in Turkish flora were investigated for their phenolic profiles and in vitro antioxidant and antidiabetic potentials
Summary
The Cistaceae family is composed of shrubs, annual or perennial herbaceous plants, and the genus Cistus is one of the broadly distributed members of this family. 50 Cistus species are distributed worldwide, and they are commonly called “rockrose” [1]. Different phenolic compounds (phenolic acids, flavonoids, proanthocyanidins) and terpenes were isolated from Cistus species, and their therapeutic benefits are generally attributed to these components [4,5]. In Turkey, five Cistus species grow naturally, i.e., C. salviifolius L., C. parviflorus Lam., C. monspeliensis L., C. laurifolius L. and C. creticus L. In the ethnobotanical records of Turkish folk medicine, various organs of Cistus species are frequently documented as a remedy. Infusions prepared from the branches of C. laurifolius, C. salviifolius and C. creticus are ingested orally against diabetes in Edremit (Balıkesir) district [7]. Decoctions prepared from the flowers of C. creticus and C. salviifolius are used internally against peptic ulcer in Marmaris (Muğla) [8], while a decoction of the unopened flower buds of C. laurifolius is used for the same purpose. In Western Anatolia, the decoction of C. laurifolius leaves is used internally against fever and stomachache and externally, via bathing, against rheumatic pain [9]
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
