Abstract
BACKGROUND: JAr and Jeg-3 choriocarcinoma cell lines are model systems for the transformed trophoblast and allow studies of phenotype and regulatory factors for particular cell functions. Both cell lines express the receptor for insulin-like growth factor-I (IGF-I). Effects of IGF-I on adhesion, proliferation and galectin-1 production in JAr and Jeg-3 cells were studied. METHODS: The effects of IGF-I on proliferation and galectin-1 production were examined by thiazolyl blue assay and cell based solid phase assay using polyclonal anti-galectin-1 antibodies. The cell adhesion assay was performed on Matrigel coated wells. Galectin-1 production and localization was examined by immunocytochemistry. RESULTS: IGF-I decreased adhesion of JAr cells to 70% of the control value (p<0.05). Cell treatment with 10 ?g/L of IGF-I significantly increased viable cell number: by 13.5% in JAr and 6% in Jeg-3. Gal-1 was immunolocalized intracellularly and associated with the cell membrane in both cell lines. Production of galectin-1 was significantly increased after treatment with IGF-I compared to control: by 7% in JAr cells and by 16% in Jeg-3 cells (p<0.05). CONCLUSION: The data showed that IGF-I affected adhesion and proliferation of choriocarcinoma cells, depending on the cell line. Both choriocarcinoma cell lines studied here produced galectin-1. The amount of galectin-1 was moderately stimulated by IGF-I.
Highlights
Choriocarcinoma, originating from the trophoblast of the human placenta, is a relatively rare malignant tumor [1]
It has been demonstrated that the expression pattern of gal-1 is altered in carcinomas, including those arising from thyroid, endometrium, head and neck, thymus, bladder, pancreas, colon, ovary [14], and trophoblast [15]
The viable cell number of JAr cells increased by 13.5% when the cells were cultured in medium containing 10 μg/L of insulin-like growth factor-I (IGF-I) (n= 6, p
Summary
Choriocarcinoma, originating from the trophoblast of the human placenta, is a relatively rare malignant tumor [1]. JAr spheroids maintain their cytotrophoblast like morphology, while BeWo and Jeg-3 spheroids expose flattened and more differentiated cells on their surfaces [2] These cell lines have an invasive phenotype, proliferate [2,3], and express receptor for the insulin-like growth factor-I (IGF-I) [4]. The aim of this study was to investigate the possible effects of IGF-I on adhesion and proliferation of JAr and Jeg-3 choriocarcinoma cells and their production of gal-1. JAr and Jeg-3 choriocarcinoma cell lines are model systems for the transformed trophoblast and allow studies of phenotype and regulatory factors for particular cell functions. Both cell lines express the receptor for insulin-like growth factor-I (IGF-I).
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