Influence of ibuprofen and its biotransformation products on different biological sludge systems and ecosystem

Abstract

Ibuprofen (IBU) is one of the frequently detected non-steroidal anti-inflammatory drugs (NSAIDs) in wastewater treatment plants (WWTPs) and aquatic environment. However, little is known about the effect of IBU and its biotransformation products (TPs) on different biological sludge systems and aquatic environment. The effects and toxicity of IBU and TPs on three biological sludge systems (i.e., activated sludge (AS), sulfate-reducing bacteria (SRB)-enriched sludge and anaerobic methanogenic (AnM) sludge systems) and aquatic environment were comprehensively evaluated through a long-term operation of three bioreactors and a series of batch experiments. Both of the SRB-enriched sludge and AnM sludge systems were not affected under a long-term exposure to IBU, based on removing organic carbon and sulfur and producing methane. This could be attributed to the high tolerance of functional microbes in the SRB-enriched sludge (e.g., genus Desulfobacter) and AnM sludge systems (e.g., genus Candidatus Methanomethylicus) for IBU. In contrast, IBU had some apparently inhibitory effects on the AS system, such as reduced organic removal efficiency and poor sludge settling. The analysis on microbial community revealed that IBU significantly inhibited the genera involved in organic degradation (e.g., genus Candidatus Competibacter) and also stimulated those genera (e.g., genus Brachymonas) to secret excess extracellular polymeric substances (EPS), which thus caused sludge bulking in the AS system. The toxicity of IBU and its TPs in the effluent of the AS system was also investigated with Vibrio fischeri bioluminescence inhibition tests and quantitative structure activity relationship (QSAR) analysis by ecological structure-activity relationship (ECOSAR) program. The results indicated that the AS system could effectively eliminate the acute toxicity of both IBU and TPs, but a potential chronic toxicity of IBU could still existed, which could be more harmful to aquatic organisms than that of its TPs. These findings provide an insight into the toxic effects of IBU and its TPs on biological sludge systems and ecosystem.