Abstract
The effect of hypoxia on the lethal response of cells exposed to single or fractionated heat treatments was examined. Chinese hamster ovary cells were exposed to graded 43/sup 0/C heat treatments in medium equilibrated with air or nitrogen (25-40 ppm O/sub 2/) plus 5% CO/sub 2/ at pH 7.25-7.4. Hypoxia was induced approx. =0.5 hr prior to exposure of the cells to the single or initial heat treatment and was maintained until the second heat treatment was terminated. The response of cells to single heat treatments was not significantly different under oxic and hypoxic conditions, yielding D/sub 0/'s of 13.5 +/- 1.4 and 14.9 +/- 1.6 min, respectively. Following an initial heat treatment which reduced survival to 10%, cells became increasingly resistant, up to ninefold, to second heat treatments administered 0 to 12 hr later. Reduction in oxygen concentration during and between heat treatments affected neither the magnitude nor the kinetics of thermotolerance development.
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