Influence of hypergravity on fish inner ear otoliths: II. Incorporation of calcium and kinetotic behaviour

Abstract

Larval siblings of cichlid fish ( Oreochromis mossambicus) were subjected to hypergravity (hg; 3g, 14 days) during development. Following the transfer to 1g (i.e., stopping the centrifuge) they were seperated into normally and kinetotically swimming individuals (the latter performed spinning movements). During hg, the animals were maintained in aquarium water containing alizarin-complexone (AC), a fluorescent calcium tracer. Densitometric measurements of AC uptake into inner ear otoliths (optical density of AC/μm 2) revealed that the kinetotic individuals had incorporated significantly more AC/calcium than the normally behaving fish. Since the amount of otolithic calcium can be taken as an approximation for otolith weight, the present results indicate that the otoliths of kinetotically swimming samples were heavier than those of the normally behaving larvae, thus exhibiting a higher absolute weight asymmetry of the otoliths between the right vs. the left side of the body. This supports an earlier concept according to which otolith (or statolith) asymmetry is the cause for kinetoses such as human static space sickness.