Abstract
In callus and tumor tissues of <i>Nicotiana tabacum</i> cultured for 39 days in media supplemented with various concentrations of hydroxyurea (1.3 x 10<sup>-4</sup> M - 1.3 x 10<sup>-3</sup> M) a decrease of DNA content (ca. 24 per cent in callus tissue and ca. 23 per cent in tumour tissue) and a decrease of RNA content (over 10 per cent and ca. 9 per cent in callus and tumour tissue, respectively) was observed. The autoradiographic method showed that a long-lasting action of this com-pound inhibits RNA synthesis. A stronger inhibitory influence of hydroxyurea upon incorporation of 3H-uridine from the incubation medium was revealed.
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