Abstract
The digestion of different chromatin preparations from calf thymus with micrococcal nuclease was studied in order to estimate the effect of high-mobility-group nonhistone chromosomal proteins 1 and 2 on the structure of chromatin. Chromatin selectively depleted of histone H1 was digested faster than chromatin depleted of H1 and most of the nonhistone proteins including HMG1 and 2. Reconstitution of the latter chromatin with purified HMG1 or 2 raised its sensitivity to the level of selectively H1-depleted chromatin. Addition of HMG1 or 2 to DNA also increased the rate of digestion. Thus HMG1 and 2 have a decondensing effect on chromatin via destabilization of the structure of its DNA. The results are discussed in view of the possible involvement of HMG1 and 2 in the structure of transcriptionally active or replicating chromatin.
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