Influence of hepatocyte growth factor in neovascularization of human glioma

Abstract

Objective To investigate the effect ofhepatoeyte growth factor (HGF) on the neovascularization of human glioma and its mechanism. Methods Seventy-two patients with gliomas,admitted to our hospital from January 2002 to June 2008,including 19 with grade Ⅰ,18 with grade Ⅱ,20 with grade Ⅲ and 15 with grade Ⅳ,were chosen in our study.HGF and CD34 expressions in the paraffin section of gliomas in these 72 patients were detected with immunohistochemistry.Fluorescently labeled HGF-siRNA was transfected into U87MG glioma cells with TurboFect siRNA Transfection Reagent as si-HGF transfection group,and normal control group and negative transfection group were employed as controls; the HGF and vascular endothelial growth factor (VEGF) expressions in these U87MG cells were detected by Western blotting; cells fiom the 3 groups were inoculated to chorioallantoic membrane (CAM), and the angiogenesis ability of these U87MG cells were determined 5 d later. Results HGF-positive rate in patients with low-grade gliomas (grade Ⅰ and Ⅱ) was significantly lower than that in patients with high-grade glioma (grade Ⅲ and Ⅳ,P＜0.05); micro-vascular density (MVD) in gliomas with positive HGF was obviously higher than that in gliomas with negative HGF (P＜0.05).As compared with those in cells of the normal control group and negative transfection group, the HGF and VEGF expressions in cells of the si-HGF transfection group were descended by 76％ and 53％,respectively.The number and area of new vessels and the neovascularization ratio in cells of the si-HGF transfection group were signficantly decreased as compared with those in cells of the normal control group and negative transfection group (P＜0.05). Conclusion Close relation exists between HGF and neovascularization of gliomas,which indicates that HGF might become a new target for future antiangiogenesis treatment. Key words: Glioma; Hepatocyte growth factor; Neovascularization