Influence of Heparin on Fibrinogen Assay by Clauss Method

Abstract

Objective: To investigate the effect of heparin on fibrinogen detection by Clauss method. Methods: A normal plasma pool (NPP) of 20 healthy people was prepared with 3.2% sodium citrate anticoagulant. For experimental group 1, samples containing different concentrations of heparin were prepared. For experimental group 2, samples of experimental group 1 were diluted twice. For experimental group 3, samples of experimental group 1 were diluted 4 times. For the control group, samples of normal saline with the same volume as heparin in experimental group 1 were prepared. The fibrinogen contents of experimental group 1, experimental group 2, experimental group 3, and control group were detected by Clauss method and prothrombin time (PT)-derived method, and the fibrinogen detection results of different groups were analyzed. Results: The trend of fibrinogen detected by Clauss method and PT-derived method in experimental group 1 was different; there was significant difference between the results of experimental group 1 and the control group (P < 0.05); there was no significant difference between the results of experimental group 2 and the control group (P > 0.05); there was no significant difference between the results of experimental group 3 and the control group (P > 0.05); there was no significant difference between the results of experimental group 2 and experimental group 3 (P > 0.05); the relative deviation between experimental group 1 and the control group was higher in high-concentration heparin sample. Conclusion: Heparin affects fibrinogen detection by Clauss method, and the effect can be reduced by sample dilution.