Abstract
We determined the effects of helium-neon (He-Ne) laser irradiation on wound healing dynamics in mice treated with steroidal and non-steroidal anti-inflammatory agents. Male albino mice, 28-32 g, were randomized into 6 groups of 6 animals each: control (C), He-Ne laser (L), dexamethasone (D), D + L, celecoxib (X), and X + L. D and X were injected im at doses of 5 and 22 mg/kg, respectively, 24 h before the experiment. A 1-cm long surgical wound was made with a scalpel on the abdomens of the mice. Animals from groups L, D + L and X + L were exposed to 4 J (cm(2))-1 day-1 of He-Ne laser for 12 s and were sacrificed on days 1, 2, or 3 after the procedure, when skin samples were taken for histological examination. A significant increase of collagen synthesis was observed in group L compared with C (168 +/- 20 vs 63 +/- 8 mm(2)). The basal cellularity values on day 1 were: C = 763 +/- 47, L = 1116 +/- 85, D = 376 +/- 24, D + L = 698 +/- 31, X = 453 +/- 29, X + L = 639 +/- 32 U/mm(2). These data show that application of L increases while D and X decrease the inflammatory cellularity compared with C. They also show that L restores the diminished cellularity induced by the anti-inflammatory drugs. We suggest that He-Ne laser promotes collagen formation and restores the baseline cellularity after pharmacological inhibition, indicating new perspectives for laser therapy aiming to increase the healing process when anti-inflammatory drugs are used.
Highlights
Morphological and biochemical studies in humans have revealed a sophisticated mechanism for skin wound healing, including replacement of the affected subcutaneous tissue with a new matrix and re-epithelization
We determined the effects of helium-neon (He-Ne) laser irradiation on wound healing dynamics in mice treated with steroidal and nonsteroidal anti-inflammatory agents
Animals from groups L, D + L and X + L were exposed to 4 J-1 day-1 of He-Ne laser for 12 s and were sacrificed on days 1, 2, or 3 after the procedure, when skin samples were taken for histological examination
Summary
Morphological and biochemical studies in humans have revealed a sophisticated mechanism for skin wound healing, including replacement of the affected subcutaneous tissue with a new matrix and re-epithelization. Injury to the skin triggers a cascade of events including inflammation and tissue remodeling, which eventually leads to total or partial restoration of the injured area [1,2]. Various aspects of this complex process have attracted the attention of researchers over the years, the factors which may hinder it. The main endogenous hormone involved in the dynamics of tissue repair is cortisol, which affects the metabolism of carbohydrates and proteins and exerts important anti-inflammatory effects [5,6]
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