Influence of Growth Phase on Bacterial Deposition: Interaction Mechanisms in Packed-Bed Column and Radial Stagnation Point Flow Systems

Abstract

The influence of bacterial growth stage on cell deposition kinetics has been examined using a mutant of Escherichia coli K12. Two experimental techniques--a packed-bed column and a radial stagnation point flow (RSPF) system--were employed to determine bacterial deposition rates onto quartz surfaces over a wide range of solution ionic strengths. Stationary-phase cells were found to be more adhesive than mid-exponential phase cells in both experimental systems. The divergence in deposition behavior was notably more pronounced in the RSPF than in the packed-bed system. For instance, in the RSPF system, the deposition rate of the stationary-phase cells at 0.03 M ionic strength was 14 times greater than that of the mid-exponential cells. The divergence in the packed-bed system was most significant at 0.01 M, where the deposition rate for the stationary-phase cells was nearly 4 times greater than for the mid-exponential cells. To explain the observed adhesion behavior, the stationary and mid-exponential bacterial cells were characterized for their size, surface charge density, electrophoretic mobility, viability, and hydrophobicity. On the basis of this analysis, it is suggested that the stationary cells have a more heterogeneous distribution of charged functional groups on the bacterial surface than the mid-exponential cells, which results in higher deposition kinetics. Furthermore, because the RSPF system enumerates only bacterial cells retained in primary minima, whereas the packed column captures mostly cells deposited in secondary minima, the difference in the stationary and mid-exponential cell deposition kinetics is much more pronounced in the RSPF system.