Abstract
Objective To determine the mechanism of Grape seed proanthocyanidin extract on a model of brain injury induced by deep hypothermic cerebral ischemia/reperfusion (I/R).Methods Sixty four-week-old C57BL6 mice were randomly and equally divided into sham operation group,model group and grape seed proanthocyanidin extract (GSPE) group.The mice were sacrificed 24 h after reperfusion for sampling of brain tissues.The expression of serine/threonine kinase (AKT) protein was detected by using Western blotting,Caspase-3 mRNA was detected by reverse transcription-polymerase chain reaction ( RTPCR) technique,and apoptosis of cerebral cells was examined by using TUNEL staning.Results The expression of p-AKT protein and Caspase-3 mRNA,and apoptotic level in model group and GSPE group were significantly increased as compared with sham operation group ( P ＜ 0.05 ).The expression of p-AKT protein [A ( 1.9 ±0.02) ＞ ( 1.6 t0.01 ),P ＜0.05 ] was increased,and expression of Caspase-3 mRNA and apoptosis were significantly decreased in GSPE group as compared with model group [ ( 0.43 t 0.02 ) ＜(0.86 ±0.03); (40.92 ±3.18)％ ＜ (79.86 ±4.68)％,P＜0.05].Conclusion The results suggest that GSPE has a protective effect against deep hypothermic cerebral I/R injury through AKT-Caspase-3 pathway. Key words: Grape seed proanthocyanidin extract; Cerebral ischemia; Reperfusion; AKT; Caspase-3
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